Figure 3
Effects of cold stress on the expression of tubulovesicle exocytosis-related factors on cultured rat organotypic gastric slices. (A) The exocytosis mechanism of tubulovesicles. Upon stimulation, tubulovesicles translocate and connect with the apical canalicular membrane, resulting in acid secretion. (B–H) Incubation at 32 °C for 3 h increased the expression of mRNAs encoding the cholecystokinin B receptor (CCKBR), histamine receptor (HRH2), transient receptor potential mucolipin-1 (TRPML1), somatostatin (SST), and somatostatin receptor 2 (SSTR2). The expression of mRNAs encoding the acetylcholine receptor (CHRM3) and carbonic anhydrase (CA2) did not change. Data are expressed as mean ± SEM (n = 7, rats). * and ** indicate p < 0.05 and 0.01, respectively, as compared to a control group incubated at 37 °C. NS, not significantly different.
