Figure 1
Generation of Mgat4d mutant mice. (A) Map of the targeted Mgat4dtm1a(KOMP)Wtsi allele in ES cells obtained from KOMP. Exon 4 is flanked by two loxP sites. LacZ and the neomycin cassettes are flanked by two Frt sites. (B) PCR of genomic DNA from Mgat4d[+/+], Mgat4d[+/−], Mgat4d[−/−] and Mgat4d[F/−]:Stra8-iCre pups to determine genotype. Primers are given in Supplementary Table S1. (C) Western blot analysis of protein extracts of germ cells purified from 28 dpp Mgat4d[+/+], Mgat4d[+/−] and Mgat4d[−/−] mice. Long and short forms of MGAT4D are identified. *is a non-specific band. Full blot in Supplementary Fig. S1. (D) Representative testis section from a mouse carrying the LacZ gene under the control of the Mgat4d promoter after staining for β-galactosidase (blue). Nuclei were stained with eosin. (E) Immunohistochemistry of representative testis sections from Mgat4d[+/−] and Mgat4d[−/−] mice of 28 dpp. The presence of MGAT4D is shown by the brown stain consistent with a Golgi localization (arrows). Nuclei were stained with hematoxylin.
