Figure 10
Differential gene interactions between Mgat4d genotype and heat shock conditions. (A) Heat maps showing DEGs either down- or up-regulated specifically in Mgat4d wild type, but not Mgat4d KO cells following heat shock, representative of significantly enriched pathways identified by EnrichR (http://amp.pharm.mssm.edu/Enrichr/). Adjusted p values and odds ratios (OR) for the respective pathways are shown. Full names of the pathways are: Single Gene Perturbations from GEO: Il10 KO mouse GSE25846 sample 3062; ARCHS4 TFs Coexp: HIF1A_human_tf_ARCHS4_coexpression; TRRUST Transcription Factors 2019: NFKB1_ human; Disease Perturbations from GEO up: Infertility due to azoospermia C1321542 mouse GSE3676 sample 151. Color scales represent gene-wise Z-scores. (B). Box plots showing expression of representative genes of the indicated pathways across genotypes and heat shock conditions. These genes were much more up-regulated by heat treatment in WT compared to mutant (KO) germ cells.
