Figure 2
Effects of heat treatment on Mgat4d[−/−] testes. (A) Representative testis sections stained with H&E. Upper panels from mice whose lower body was submerged for 25 min at 33 °C and lower panels from mice treated similarly at 43 °C. Arrows indicate enlarged cells, arrow heads show vacuoles in germ cells. (B) Representative epididymis sections from a Mgat4d[−/−] male treated at 33 °C (upper) or 43 °C (lower) and stained with H&E. Arrows in the 43 °C sample indicate pyknotic cells in the tubule lumen. (C) Quantification of different tubule categories in testis sections from heat-treated (43 °C) Mgat4d[+/−] and Mgat4d[−/−] males. Positive tubules were counted as those with at least one cell of radius ≥10 μm; large vacuoles were tubules with at least one vacuole ≥10 μm; small vacuoles, pyknotic cells were tubules with at least one vacuole of radius <10 μm or tubules containing pyknotic cells; undamaged tubules were tubules with no apparent damage. Mice were from an aged cohort (592–596 days) of Mgat4d[+/−](n = 2) and Mgat4d[−/−](n = 5) mice. Thirty (30) tubules were counted in one section per mouse. Student’s t test (two-tailed, unpaired) **p < 0.01; *p < 0.05.
