Table 1 Analysis of variance for the fitted quadratic polynomial model for recovery of CUI, CUB, and CUI + B.

From: Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Sourcea

CUI

CUB

CUI + B

SS

df

MS

F-value

p-value

SS

df

MS

F-value

p-value

SS

df

MS

F-value

p-value

Model

6.92

20

0.3461

2.86

0.0069**

4.04

20

0.2021

3.17

0.0036**

20.14

20

1.01

3.61

0.0014**

A-SS ratio

0.1887

1

0.1887

1.56

0.2231

0.2647

1

0.2647

4.15

0.0524

0.9004

1

0.9004

3.23

0.0844

B-RPM

0.0953

1

0.0953

0.7885

0.3830

0.0060

1

0.0060

0.0935

0.7623

0.0536

1

0.0536

0.1922

0.6649

C-PS

3.27

1

3.27

27.03

 < 0.0001**

2.06

1

2.06

32.24

 < 0.0001**

10.51

1

10.51

37.70

 < 0.0001**

D-min

0.1090

1

0.1090

0.9018

0.3514

0.0331

1

0.0331

0.5192

0.4779

0.2624

1

0.2624

0.9412

0.3413

E- Temp

0.1476

1

0.1476

1.22

0.2797

0.0538

1

0.0538

0.8425

0.3675

0.3795

1

0.3795

1.36

0.2543

AB

0.0068

1

0.0068

0.0566

0.8138

0.0125

1

0.0125

0.1959

0.6619

0.0379

1

0.0379

0.1358

0.7156

AC

0.7345

1

0.7345

6.08

0.0209*

0.6365

1

0.6365

9.97

0.0041**

2.74

1

2.74

9.82

0.0044**

AD

0.0037

1

0.0037

0.0307

0.8623

0.0105

1

0.0105

0.1644

0.6886

0.0017

1

0.0017

0.0062

0.9380

AE

0.0000

1

0.0000

0.0003

0.9858

0.0014

1

0.0014

0.0215

0.8845

0.0019

1

0.0019

0.0067

0.9352

BC

0.0026

1

0.0026

0.0215

0.8846

0.0060

1

0.0060

0.0947

0.7608

0.0007

1

0.0007

0.0026

0.9600

BD

0.0110

1

0.0110

0.0912

0.7652

0.0080

1

0.0080

0.1258

0.7258

0.0379

1

0.0379

0.1358

0.7156

BE

0.0802

1

0.0802

0.6631

0.4231

0.0740

1

0.0740

1.16

0.2919

0.0001

1

0.0001

0.0004

0.9834

CD

0.3749

1

0.3749

3.10

0.0905

0.0909

1

0.0909

1.42

0.2438

0.8352

1

0.8352

3.00

0.0958

CE

1.02

1

1.02

8.45

0.0075**

0.2984

1

0.2984

4.67

0.0404*

2.42

1

2.42

8.70

0.0068*

DE

0.0441

1

0.0441

0.3649

0.5512

0.0295

1

0.0295

0.4622

0.5028

0.1458

1

0.1458

0.5229

0.4763

A²

0.2348

1

0.2348

1.94

0.1757

0.0130

1

0.0130

0.2040

0.6554

0.1372

1

0.1372

0.4922

0.4894

B²

0.1916

1

0.1916

1.59

0.2196

0.0275

1

0.0275

0.4310

0.5175

0.0739

1

0.0739

0.2652

0.6111

C²

0.1407

1

0.1407

1.16

0.2909

0.2681

1

0.2681

4.20

0.0511

0.7972

1

0.7972

2.86

0.1033

D²

0.0107

1

0.0107

0.0888

0.7682

0.2564

1

0.2564

4.02

0.0560

0.1622

1

0.1622

0.5819

0.4527

E²

0.1797

1

0.1797

1.49

0.2342

0.0008

1

0.0008

0.0121

0.9134

0.1569

1

0.1569

0.5628

0.4601

Residual

3.02

25

0.1209

  

1.60

25

0.0638

  

6.97

25

0.2788

  

Lack of Fit

2.30

20

0.1149

0.7939

0.6804

1.20

20

0.0599

0.7540

0.7067

5.40

20

0.2701

0.8614

0.6377

Pure Error

0.7238

5

0.1448

  

0.3973

5

0.0795

  

1.57

5

0.3136

  

Cor Total

9.95

45

   

5.64

45

   

27.11

45

   
  1. aIndependent variables, SS- sum of squares; MS – mean square, df – degree of freedom.
  2. *Significant at p-value < 0.05.
  3. **Significant at p-value < 0.01.
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