Figure 2
From: CNN3 acts as a potential oncogene in cervical cancer by affecting RPLP1 mRNA expression
Stable CNN3 knockdown inhibits the growth and metastasis of xenografts of cervical cancer in vivo. (A) Immunoblot analysis of CNN3 protein in SiHa cells with sh-NC and sh-CNN3 lentivirus. The grouping of blots cropped from same parts of the same gel. Full-length blots are presented in Supplementary Fig. S4. (B,C) SiHa cells with sh-NC and sh-CNN3 were injected into nude mice (approximately 2.5 × 106 cells, n = 6 in each group). Xenografts were extracted at week 7 when mice were sacrificed under anesthesia. The volume of subcutaneous tumours was tested weekly and calculated using the following formula: V = (L × W2)/2, mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001. (D,E) SiHa cells (approximately 2.5 × 106 cells, n = 6 in each group) were intravenously injected into NOD/SCID mice via tail vein. The metastasis was weekly detected by measuring the bioluminescence with an IVIS Lumina LT system. The images and corresponding luminescence intensity at week 1 and week 12 after injection were displayed. Data represent mean ± SD (*P < 0.05). (F,G) Lungs were extracted at week 12 when mice were sacrificed under anesthesia. Lung metastases with white circular nodules (black arrow indicated) and corresponding numbers of metastatic nodules were showed. Data represent mean ± SD (*P < 0.05).
