Figure 4

RPLP1 modulates the viability and activity of cervical cancer cells. (A,B) Cell proliferation of SiHa and CaSki cells were detected by CCK8 assays at 24, 48, 72, 96, 120 h after transfection with NC-plasmid, RPLP1-plasmid, si-NC, si-RPLP1#1, and si-RPLP1#2, respectively. Data represent mean ± SEM (n = 3). **P < 0.01, ***P < 0.001, ^###P < 0.001. (C,D) Representative images of colony-formation assays for the proliferation in SiHa and CaSki cells with RPLP1 up-regulation and down-regulation are shown (left panels), respectively. Quantification and statistics of colony area percentage of each well are shown (right graphs), mean ± SEM (n ≥ 3). *P < 0.05, **P < 0.01. (E) Representative images of boyden chamber migration and invasion assays for the migration and invasion of SiHa and CaSki cells with NC-plasmid, RPLP1-plasmid (left panel), si-NC, si-RPLP1#1 and si-RPLP1#2 (right panel) for 24 h, respectively. Representative images (scale bars, 100 μm) and corresponding number (SiHa) or area (CaSki) of migrated (or invaded) cells from three independent experiments are shown, mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.