Figure 5
From: CNN3 acts as a potential oncogene in cervical cancer by affecting RPLP1 mRNA expression
RPLP1 participates in CNN3-modulated malignant behaviours in cervical cancer cells. SiHa and CaSki cells were co-transfected with si-NC plus NC-plasmid, si-CNN3#1 plus NC-plasmid and si-CNN3#1 plus RPLP1-plasmid. (A) 48 h post-transfection, whole-cell lysates were obtained to analyze CNN3 and RPLP1 protein level with immunoblot assays. Representative results are shown. Full-length blots are presented in Supplementary Fig. S7. (B) CCK8 assays were applied to detect the proliferation of SiHa and CaSki cells. Data are shown of three independent experiments, mean ± SEM. ***P < 0.001, si-CNN3#1 plus NC-plasmid group vs si-NC plus NC-plasmid group; ##P < 0.01, ###P < 0.001, si-CNN3#1 plus RPLP1-plasmid group vs si-CNN3#1 plus NC-plasmid group. (C) Transwell migration and invasiveness assays were performed to detect cell migration and invasion. Left, representative images (scale bars, 100 um). Right, the corresponding histograms representative of three independent experiments (data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001).
