Figure 3
From: “UniCAR”-modified off-the-shelf NK-92 cells for targeting of GD2-expressing tumour cells
Structure of scFv- and IgG4-based TMs for redirection of UniCAR NK-92 cells. (A) The single-chain fragment variable (scFv)-based TM specific for GD2 consists of the variable domains of the antibody heavy (VH) and light (VL) chains connected to the E5B9 epitope and a polyhistidine tag via glycine-serine linkers. (B) To generate an IgG4-based TM, the GD2-specific scFv domain was fused to the hinge, CH2 and CH3 constant regions of human IgG4, with the E5B9 epitope and a polyhistidine tag placed C-terminally of the CH3 region. The amino acid residues forming the peptide linkers between the different domains are indicated. Cysteine residues within the hinge region allow dimerization via disulfide bonds and the formation of a homodimeric IgG4-based TM. SP, signal peptide.
