Figure 8
From: “UniCAR”-modified off-the-shelf NK-92 cells for targeting of GD2-expressing tumour cells
IFNγ release by UniCAR NK-92 cells redirected by α-GD2 TMs. UniCAR 28/ζ NK-92 cells were cultured with JF Luc neuroblastoma cells at an E:T ratio of 5:1 in the presence or absence of α-GD2 scFv or IgG4 TMs. EGFP-expressing (Vector control) or UniCAR stop (lacking signalling domains) NK-92 cells were included as controls. After 4 hrs, supernatants were collected and IFNγ concentrations were determined by ELISA. Results are shown as mean ± SD of two independent experiments.
