Figure 3
Recombinant protein expression of Hsp27, HspA1, and hCNTF in a batch fermentation process with 2xYTPG rich medium (hCNTF-1) for 4 hours, and hCNTF in continuous fermentation process with EnBase medium23 (hCNTF-2) for 24 hours. All fermentations were done in high yield flasks45 at 30 °C and 225 rpm (1” amplitude). The SDS-PAGE compared the amount of protein per cell before induction (−) and after induction with IPTG (+). The prepared target protein is indicated by an asterisk.
