Table 1 Shake flask cultivation samples.

Protein	Media	Hours after Induction	OD₆₀₀	Growth Phase
HspA1	2xYTPG	0	0.43	Log
		1¹	0.89	Log
		2	1.89	Log
		3	3.20	Log
		4¹	3.65	Log
		5	6.10	Stationary
		6¹	6.80	Stationary
Hsp27	2xYTPG	0	0.54	Log
		1	1.03	Log
		2	1.32	Log
		3	1.54	Log
		4	1.70	Log
		5	1.91	Log
		6	2.15	Log
hCNTF	2xYTPG	−1	0.31	Lag
		0	0.49	Lag
		1¹	0.82	Log
		2	1.05	Log
		3¹	1.79	Log
		4¹	3.32	log
hCNTF	EnBase	0¹	11.7²	Log
hCNTF	EnBase	24¹	43.8²	Log
—	2xYTPG	4^1,3	3.04	Log
—	EnBase⁴	0¹	—	—
—	EnBase⁴	24¹	—	—

(1) Samples also used for SERS measurements; (2) Cell dry Weight (CDW) was determined and values calculated to OD₆₀₀ as previous described;²⁴ (3) These cells acting as negative control were not induced, and this sample was taken 3.5 hours after inoculation (see Fig. 2). The OD₆₀₀ at time-point zero, normalized to time-of-induction of the other samples in Fig. 2 was 0.51; (4) EnBase media without cells was used as a blank for the CDW determination (CDW at 0 hours was 0 and at 24 hours after induction of the hCNTF EnBase culture was 12.5 g L⁻¹).

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