Table 2 Tentative assignments of Raman/SERS bands.

From: Assessment of recombinant protein production in E. coli with Time-Gated Surface Enhanced Raman Spectroscopy (TG-SERS)

Measured Raman/SERS bands [cm⁻¹]	Literature reference Raman/SERS bands [cm⁻¹]	Tentative assignments (interpretation of Raman/SERS bands)	Origin/Category	reference
530	510–550	S-S bond stretching	Proteins containing S-S bridges	^18,46
550–580	560–580	Ring and CH-deformation	Carbohydrate, yeast and growth medium	²⁷
613	620	Aromatic amino acids	Phenylalanine	⁴⁶
710–735	732	DNA /CH₂ rocking	Adenine or cAMP, growth medium	²⁷
820–860	830–860	Aromatic amino acids, NC minor influence	Tyrosine and/or NC	^13,18,46
918–925	924–943	N−C_α−C stretch	Valine	¹⁸
942–950	957–964	C = C deformation	DNA (guanine)	^27,47
1008–1030	1000, 1030	Aromatic amino acids, NC minor influence	Phenylalanine and/or NC	this work^46,48
1050	1033	C−H plane bending of aromatic compounds	Phenylalanine, tryptophan, tyrosine	this work⁴⁹,
1130–1195	1134–1160	C−N and C−C stretch	Carbohydrate in medium	²⁷
1170–1195	1170–1200	Aromatic amino acids	Tyrosine	⁴⁸
1232–1242	1232–1250	N−H and C−H bend	Amide III (β-sheet)	^18,50
1295–1336	1300−1345	N−H and C−H bend	Amide III (α-helix)	^13,50
1365–1367	1320–1340	DNA, Nucleotide	Adenine (AMP)	²⁷
1398–1405	1390–1398	Symmetric deformation of CH₃ or overtone of Amide V	Amide V or Peptide, growth medium	^13,51
1423	1420–1480	DNA or CH₂ deformation	Adenine, Guanine	⁴⁷
1450	1444–1450	CH₂ deformation	Lipid	^18,48
1505–1515	—	CH₂ deformation	Carbohydrate in growth medium	this work
1520–1550	1550	N–H bend and C–N stretch	Amide II	⁴⁶
1590	1582–1590	Aromatic amino acids	Tryptophan or Tyrosine	⁴⁶
1605	1609	Aromatic amino acids	Phenylalanine or Tyrosine	this work⁴⁶
1624–1627	1617	Aromatic amino acids or part of C = C stretch	Tryptophan or Tyrosine	^18,47
1650–1657	1660–1677	C = O stretch	Amide I	^18,46

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Table 2 Tentative assignments of Raman/SERS bands.

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