Figure 3
From: Regulation of bone metabolism by megakaryocytes in a paracrine manner
Stimulation of calvaria bone formation by MK CM. 30× Megakaryocyte (MK) and pro-megakaryocyte (pro-MK) conditioned media (CM), and non-CM were injected on the right side of the calvaria in 5-week-old female C57BL/6 mice for 4 weeks. PBS was injected on the left side of the calvaria as a negative control. Hematoxylin and eosin staining (a), and alkaline phosphatase immune histochemical (ALP, brown) and tartrate-resistant acid phosphatase (TRAP, purple) stainings (b) were performed with the sections of calvaria. (c) The osteoblastic surface percentage (OB.S/BS, %) and number per bone surface (OB.S/BS, N/mm), and the osteoclastic surface percentage (OC.S/BS, %) and number per bone surface (OC.S/BS, N/mm), were shown. (d) The width of the calvaria was quantitated by the average of 4 spots with the same interval (the indicated lines) of the midline between the sagittal suture and the site of muscle attachment. Arrows indicates osteoid lines as references of calvaria width measurements. Data are presented as mean ± SEM. *P < 0.05 vs. non-CM (α-MEM media); #P < 0.05 vs. pro-MK CM. Scale bar, 50 μm.
