Figure 5

Effect of hydrostatic pressure on matrix metalloproteinase production by GBM adherent and neurosphere cells. (a) Conditioned media of cells exposed to hydrostatic pressure for 48 h were analysed by gelatin zymography. (b) Densitometric quantitation of MMP-2 and MMP-9 produced in the conditioned medium. (c) Effect of hydrostatic pressure on MMP-2 and MMP-9 mRNA expression. All results are expressed as mean ± SEM of n = 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student t test.