Figure 4 | Scientific Reports

Figure 4

From: Bioevaluation of superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with dihexadecyl phosphate (DHP)

Figure 4

Prussian blue staining was performed for quantitative evaluation of the SPIONs uptake using the Iron Staining Kit (Sigma Aldrich, MO, USA). TCam-2 and SW1353cells were seeded at density of 50,000 and 100,000 cells (respectively) per well on 12-well plate. After 24 h incubation the SPION-DHP and SPION-DMSA were applied to cells in complete growth medium to final concentration of 0.1 mg/mL. Prussian blue staining was performed 24 h after iron oxide nanoparticles administration.

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