Figure 4 | Scientific Reports

Figure 4

From: Neutrophil extracellular trap-enriched supernatants carry microRNAs able to modulate TNF-α production by macrophages

Figure 4

Extracellular miRNA carriers are present in NETs. NET induced by PMA (A,C,E) or fixed L. amazonensis promastigotes (La; ratio of 5 parasites/neutrophil) (B,D and F) were immunostained with Argonaute-2 (Ago-2), Apoliprotein-AI (Apo-AI), Elastase (Ela), DNA/Histone H1 (DNA/H) and DAPI followed by Alexa fluor 488 or 546 secondary antibodies. Dapi staining images merged with differential interference contrast are shown in Leishmania-treated samples showing NET ensnared promastigote (inserts, arrows). Bars: 10 μm.

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