Figure 1 | Scientific Reports

Figure 1

From: Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4

Figure 1

qRT-PCR characterization of the time-course of lateral plate mesoderm differentiation. Gibco hiPSCs were cultured either in 2D on Matrigel (black triangles) or 3D in fibrin (red squares) in APEL2 + GSKi for the indicated duration, and samples were quantitated with qRT-PCR. Data is presented as mean ± SEM of the log2 fold change in expression relative to the ACTB housekeeping control and the day 0 control, aggregated from at 4 independent experiments. Data represent the time-course of relative expression of pluripotency genes NANOG (A), and POU5F1 (B), primitive streak genes TBXT (C) and SOX17 (D), lateral plate mesoderm genes GATA4 (E) and FOXF1 (F), limb bud mesenchyme genes PITX1 (G) and TBX5 (H), the paraxial mesoderm gene PDGFRA (I), and the intermediate mesoderm gene MEOX1 (J).

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