Figure 6

qRT-PCR characterization of the influence of 20 µM thalidomide on hiPSC LPM differentiation. Gibco hiPSCs were differentiated in APEL2 + GSKi for 2 days in the presence of 20 µM thalidomide or 0.1% DMSO. Data represent the mean ± log2 fold change, normalized to the ACTB control and the DMSO control via the 2^−ΔΔCT method, relative to DMSO control from 4 independent experiments. Asterisks denote statistical significance via 2-tailed Student’s t-test relative to a nominal mean log2 fold change of ‘0’ (α = 0.05). PS = primitive streak; LPM = lateral plate mesoderm.