Table 1 Primary and secondary antibody pairs used for immunofluorescence staining of hiPSCs.
Primary Antibody | Dilute | Secondary Antibody | Dilute |
|---|---|---|---|
Goat anti-FOXF1 (R&D Systems AF4798) | 1:500 | Donkey anti-Goat IgG AF488 (Abcam ab150129) | 1:400 |
Mouse anti-NANOG (Abcam ab14959) | 1:200 | Donkey anti-Mouse IgG AF647 (Abcam ab150107) | 1:400 |
Mouse anti-SALL4 (Santa Cruz Clone EE-30) | 1:250 | Donkey anti-Mouse IgG AF647 | 1:400 |
Rat Anti-Oct4 (ThermoFisher Clone EM92) | 1:250 | Donkey Anti-Rat IgG DyLight 550 (Thermo SA5-10027) | 1:1000 |
Rabbit Anti-T (Abcam Clone EPR18113) | 1:500 | Donkey Anti-Rabbit IgG AF790 (Thermo A11374) | 1:1000 |
