Figure 3

Nuclear-encoded plastid rpl32 gene duplication events. (A) ML gene tree based on the nuclear-encoded rpl32 nucleotide sequences. The numbers after each species indicate the paralogs of rpl32. Bootstrap support values > 70% are shown on the branches. (B) Phylogenetic relationships within Aconitum. Ancestral gene duplications are first followed by a speciation and then by asymmetric gene losses. Three lineages have one or two ancestral paralogs. (C) Histograms of the dS distribution of the nuclear-encoded rpl32 homologs derived from recent and ancient GD events. The dS distribution was fitted using Gaussian mixture models. The dS peaks corresponding to each GD. (D) Comparison of sequence divergence between homologs from two different GD events. The figures were constructed in IQ-TREE v1.6.2 (https://iqtree.org), R v3.4.2 (https://r-project.org) and InkScape v0.92.2 (https://inkscape.org).