Figure 1
From: Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool
Giant ER vesicle formation (a) Schematic view. Sey1p-expressing cells are cultured and fractionated by multiple centrifugation steps to purify ER membranes. Sey1p-containing ER membranes are incubated with GTP to yield giant ER vesicles (GERVs), usable in confocal microscopy and other techniques. (b) Subcellular fractionation. Ratios of marker signals for different cellular organelles in the final relative to the starting material show enrichment of ER membrane and depletion of other organelle membranes. (c) GERV formation. Schematic view (top) and confocal fluorescence images (bottom) of GERV formation in the presence of 5 mM GTP. (d) Representative image of GERV formation with reconstituted Bet1p-mCherry. Membranes in (c) and (d) are fluorescently labeled with FM1-43.
