Figure 3
From: Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool
Immunostaining and confocal microscopy of the translocon Sec61p located in GERVs. (a) Sec61p was stained using anti-Sec61p (from rabbit) as the primary antibody and red fluorescent Alexa-647 labeled anti-rabbit as the secondary antibody. Antibodies were added sequentially to the GERVs (see SI). Panel (b) shows the corresponding lipid bilayer labeling with FM1-43 (green) and (c) an overlay of both images. (d) Western blot of purified microsomes with anti-Sec61p anti-rabbit-HRP antibodies (see Fig. S1b for more lanes). Panels (e,f) display GERVs, which underwent the same immunostaining procedure as in (a–c), with the exception that the primary anti-Sec61p antibody was omitted.
