Figure 5
From: Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool
GERV containing a low density of Bet1p-sfGFP for FCS and a high density of Bet1-mCherry for imaging. (a) Confocal slice at the equator. (b) Confocal slice at the top, where the FCS focus is positioned. (c) Representative example of an FCS curve, measured at the top of a GERV, and the corresponding fit.
