Figure 1 | Scientific Reports

Figure 1

From: Responsiveness of various reservoir species to oral rabies vaccination correlates with differences in vaccine uptake of mucosa associated lymphoid tissues

Figure 1

Spatio-temporal resolution of SAD L16 GFP infection in the t. palatina of foxes at day 1–4 post inoculation. (a) Genome organisation of the virus construct SAD L16 GFP. (b) Detection of virus infected cells in 150 µm vibratome slices by GFP auto-fluorescence (green) and immunostaining for RABV nucleoprotein N (red). Blue: Nuclei stained with Hoechst 33342. Top: Mosaic overview images generated from confocal tile scans performed with at low magnification (20x objective). Bottom: details from mosaic images shown. (c) Higher resolution images of individual infection foci at days 2 and 3 pi. Shown are single optical slices (left side) and maximum z-projections of confocal z-stacks.

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