Figure 5

Positivity rates of tissue samples tested by RT-qPCR for the presence of viral RNA. (a) The columns show the percentage of positive samples per species and tissue. For each species, all time points were summarised (5 time points × 2 animals, n = 10). For better orientation, dotted horizontal lines were drawn at 50% and 30%. (b) Mean positivity rates for viral RNA as demonstrated by RT-qPCR. Species were grouped according to their assumed susceptibility to oral vaccination in responsive (fox-raccoon dog-mongoose = green) and refractory (raccoon-dog-skunk = gray) species. For each group, mean rates of RT-qPCR positive tissues (mean ± s.d.; n ≥ 2) are shown. Analyses of significance in the differences between two means were calculated by two-way ANOVA followed by Šidák’s multiple comparison test. Differences between two means with p < 0.01 were considered highly significant (**). Tissue abbreviation: Lnn. mand.- lymphnodi mandibulares, Ln. paro. – lymphnodus parotideus, Ln. retro. – lymphnodus retropharyngealis, ling. ground – lingual ground, T. palat. – tonsilla pallatina (elsewhere referred to as t. palatina), T. phary. – t. pharyngea,.