Table 2 Detection of vaccine virus (ct values) in oral swabs by RT-qPCR and RTCIT.

From: Responsiveness of various reservoir species to oral rabies vaccination correlates with differences in vaccine uptake of mucosa associated lymphoid tissues

Time p.i.

2 h

4 h

1 d

2 d

3 d

4 d

10 d

Time p.i.

2 h

4 h

1 d

2 d

3 d

4 d

10 d 

RT-qPCR (ct-values) and virus isolation in RTCIT

Fox_01

22.2

29.2

—

nd

nd

nd

—

RC_01

22.2

25.4

33.8

nd

nd

nd

—

Fox_02

22.2

33.2

—

nd

nd

nd

—

RC_02

26.6

34.2

—

nd

nd

nd

—

Fox_03

19.6

29.9

—

nd

nd

28.0

 

RC_03

30.3

31.0

—

nd

nd

30.7

 

Fox_04

22.8

24.2

—

nd

nd

34.8

 

RC_04

27.5

31.4

32.0

nd

nd

—

 

Fox_05

21.1

29.6

—

nd

—

  

RC_05

26.0

29.3

—

nd

—

  

Fox_06

20.2

30.9

—

nd

32.6

  

RC_06

31.6

31.8

—

nd

—

  

Fox_07

21.4

33.4

—

—

   

RC_07

33.8

29.4

—

—

   

Fox_08

27.4

33.5

—

—

   

RC_08

28.5

30.5

—

—

   

Fox_09

27.8

37.9

36.1

    

RC_09

27.2

27.7

—

    

Fox_10

24.3

32.6

35.2

    

RC_10

31.1

29.7

34.5

    

RD_01

28.6

30.4

—

nd

nd

nd

—

Dog_01

32.0

31.7

—

nd

nd

nd

—

RD_02

30.9

32.6

—

nd

nd

nd

—

Dog_02

32.4

33.3

35.4

nd

nd

nd

—

RD_03

28.5

—

—

nd

nd

—

 

Dog_03

30.3

31.5

32.8

nd

nd

—

 

RD_04

26.0

—

—

nd

nd

—

 

Dog_04

33.9

36.2

—

nd

nd

37.4

 

RD_05

27.1

36.5

—

nd

—

  

Dog_05

32.4

35.3

—

nd

—

  

RD_06

34.9

—

—

nd

—

  

Dog_06

33.8

33.0

—

nd

—

  

RD_07

28.6

—

—

—

   

Dog_07

32.0

30.4

37.0

—

   

RD_08

26.0

30.1

—

—

   

Dog_08

31.3

34.4

—

—

   

RD_09

28.7

—

—

    

Dog_09

30.0

34.4

37.4

    

RD_10

30.9

32.2

—

    

Dog_10

27.0

30.2

—

    

MG_01

29.5

—

—

nd

nd

nd

—

SK_01

36.1

35.2

—

nd

nd

 nd

—

MG_02

30.0

—

—

nd

nd

nd

—

SK_02

30.2

32.8

36.0

nd

nd

 nd

—

MG_03

—

—

—

nd

nd

—

 

SK_03

—

35.0

—

nd

nd

—

 

MG_04

29.3

—

—

nd

nd

—

 

SK_04

—

36.5

—

nd

nd

—

 

MG_05

28.2

—

32.8

nd

—

  

SK_05

30.7

34.7

—

nd

—

  

MG_06

21.9

33.5

—

nd

35.5

  

SK_06

30.0

37.4

37.0

nd

—

  

MG_07

20.0

30.5

—

—

   

SK_07

36.7

—

36.0

—

   

MG_08

27.8

—

—

—

   

SK_08

—

—

35.7

—

   

MG_09

30.6

35.4

28.8

    

SK_09

32.4

—

—

    

MG_10

28.1

—

36.9

    

SK_10

—

31.3

—

    
  1. All animals received 108.0 FFU/mL by direct oral instillation. −: negative; nd: not determined; blank space: animals already euthanised. Oral swabs, which were positive for RABV RNA in RT-qPCR screenings, were tested for infectious virus by RTCIT. Samples for that infectious vaccine virus could be isolated are marked in bold. Animal abbreviation: RD – raccoon dog, MG – mongoose, RC – raccoon, SK – skunk.
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