Figure 4
Comparable FRET between MB- and NBD-fluorescent lipids in V and T liposomes. (A–D) Fluorescence emission spectra of V (A,C) or T (B,D) liposomes containing 1.5% (A,B) or 0.5% (C,D) MB- and NBD-labeled lipids before (red traces) or after (black traces) addition of βOG. (E) The observed FRET efficiency was plotted against the percentage of MB- and NBD-fluorescent lipids in V (black dots) or T (red dots) liposomes. Curves show fits to the exponential function f = y0 + a * (1 − exp(−b * x)), where f is the FRET efficiency, x is the percentage of fluorescent lipids and the fitted parameters were: y0 = 21.9, a = 77.2 and b = 1.83 for V liposomes, and y0 = 27.3, a = 72.1 and b = 1.58 for T liposomes.
