Figure 3
From: Intra-islet GLP-1, but not CCK, is necessary for β-cell function in mouse and human islets
CCK gene expression is regulated by glucose. (A) mRNA levels of cholecystokinin (Cck), and CCK receptors (Cckar and Cckbr) in islets isolated from lean or obese mice. (B) CCK, CCKAR, and CCKBR in isolated human islets. mRNA levels in lean mouse (C) and human islets (D) after 24 h culture in high glucose (25 mM glucose). (E) Cck in lean mouse islets after 24 h culture in high glucose in presence of 200 nM exendin-(9–39) (HG + Ex9). (F) Sulfated cholecystokinin (CCK) released into the media during culture of human islets with 10 µM 8-CPT-cAMP. Significance was tested by Student’s t-test (B,C,F) and one-way ANOVA follow by Bonferroni’s post-hoc test (D). Data are mean ± SEM (n = 5 for mouse and n = 2 for human). *P < 0.05.
