Figure 4
From: Intra-islet GLP-1, but not CCK, is necessary for β-cell function in mouse and human islets
Paracrine CCK signaling is not required for ex vivo GSIS. (A) Sulfated cholecystokinin (CCK) released into the media during islet culture. (B) Insulin secreted from batches of 5 islets after 1 h incubation at 3 or 15 mM glucose (3 G and 15 G, respectively) or 15 G in the presence of 1 µM proglumide, a non-selective CCK receptor antagonist. Insulin values were normalized to the islet DNA content from each batch (total of 9–21 batches/condition). Significance was tested by two-way ANOVA and Bonferroni’s post-hoc test. Data are mean ± SEM of 3–7 islet. preparations.
