Figure 3

Western blots of extracts of allergenic plants probed with rabbit BR84 anti-SmSEA antibodies eluted from a >90 kDa D. farinae molecule. (a) Eluted antibodies were used to probe: 1, D. farinae (44 µg/lane); 2, latex (157.5 µg/lane); 3, tomato (188 µg/lane); 4, peanut (167 µg/lane); 5, avocado (220 µg/lane); 6, kiwi fruit (94 µg/lane). (b) The same as (a), but after treatment with 10 mM Na-metaperiodate for one hour prior to application of primary antibody. M = protein molecular size marker.