Figure 4

Western immunoblots of P. australasiae and S. mansoni extracts probed with rabbit 1025Z anti-SmSEA IgG antibodies eluted from ~85 kDa and ~88 kDa P. australasiae molecules. (a) Eluted antibodies were used to probe: 1, P. australasiae extract (200 µg/lane); 2, S. mansoni SEA (20 µg/lane); 3, S. mansoni worm homogenate (20 µg/lane); 4, S. mansoni cercariae homogenate (20 µg/lane). (b) The same as (a) but after treatment with 10 mM Na-metaperiodate for one hour. M = protein molecular size marker. Supplementary Figs. S6 and S7 show uncropped blot images for lanes 1–4 of panels (a,b).