Figure 2
From: H-Ferritin is essential for macrophages’ capacity to store or detoxify exogenously added iron
Gene expression is altered in Fth1+/+ and Fth1−/− BMDM upon treated with IFNG plus LPS. Fth1+/+ (black) and Fth1−/− (grey) BMDM were treated with IFNG plus LPS, for 12, 24 or 72 hours. Gene expression for the genes of interest (a) Fth1, (b) Ftl, (c) Slc40a1, (d) Hmox1, (e) Tfrc and (f) Nos2a were normalized to the level of Hprt1. Data are shown as fold change (mean ± SD) relative to the non-treated Fth1+/+ cells, calculated with the 2−ΔΔCT method, of at least three independent experiments. Statistical analysis was obtained by two-way ANOVA, *p < 0.05, **p < 0.01 and ***p < 0.001.
