Table 4 Primers sequences.
From: H-Ferritin is essential for macrophages’ capacity to store or detoxify exogenously added iron
Gene and nomenclature | Primer | Sequence |
|---|---|---|
Hypoxanthine Phosphoribosyltransferase (Hprt) | Hprt forward | 5′-GGTGGAGATGATCTCTCAAC-3′ |
Hprt reverse | 5′-TCATTATAGTCAAGGGCATATCC-3′ | |
H-ferritin (Fth1) | Fth1 forward | 5′-GCTGAATGCAATGGAGTGTGCA-3′ |
Fth1 reverse | 5′-GGCACCCATCTTGCGTAAGTTG-3′ | |
L-ferritin (Ftl) | Ftl forward | 5′-ACCTACCTCTCTCTGGGCTT-3′ |
Ftl reverse | 5′-TGGCTTCTGCACATCCTGGA-3′ | |
Ferroportin (Slc40a1) | Slc40a1 forward | 5′-TTGGTGACTGGGTGGATAAGAATGC-3′ |
Slc40a1 reverse | 5′-CGCAGAGGATGACGGACACATTC-3′ | |
Heme oxygenase 1 (Hmox1) | Hmox1 forward | 5′-GCCACCAAGGAGGTACACAT-3′ |
Hmox1 reverse | 5′-GCTTGTTGCCCTCTATCTCC-3′ | |
Transferrin receptor (Tfrc) | Tfrc forward | 5′-GCAGCATTGGTCAAAACATGG-3′ |
Tfrc reverse | 5′-GCTTTGGGCATTTGCAACCC-3′ | |
Inducible nitric oxyde synthase (Nos2a) | Nos2a forward | 5′-ACATCGACCCGTCCACAGTAT-3′ |
Nos2a reverse | 5′-CAGAGGGGTAGGCTTGTCTC-3′ | |
Arginase 1 (Arg1) | Arg1 forward | 5′-CTCCAAGCCAAAGTCCTTAGAG-3′ |
Arg1 reverse | 5′-CGAGCTGTCATTAGGGACATCA-3′ | |
Tumor necrosis factor (Tnf) | Tnf forward | 5′-CCGTCAGCCGATTTGCTATCT-3′ |
Tnf reverse | 5′-CGGACTCCGCAAAGTCTAAG-3′ | |
Superoxide Dismutase (Sod2) | Sod2 forward | 5′-GTCGCTTACAGATTGCTGCCTGCT-3′ |
Sod2 reverse | 5′-GTGCTCCCACACGTCAATCCC-3′ | |
Catalase (Cat) | Cat forward | 5′-TCACCGGCACATGAATGGCTATGGA-3′ |
Cat reverse | 5′-TGCCCTGGTCGGTCTTGTAATGGA-3′ | |
Thioredoxin reductase (Trxr) | Trxr forward | 5′-CAGGGTGACTGCTCAATCCACAAAC-3′ |
Trxr reverse | 5′-CTCTTCCTACCGCCAGCAACACTG-3′ | |
Glutamate-cysteine ligase catalytic subunit (Gclc) | Gclc forward | 5′-TCACCGGCACATGAATGGCTATGGA-3′ |
Gclc reverse | 5′-TGCCCTGGTCGGTCTTGTAATGGA-3′ |
