Figure 6
From: Abrogation of type-I interferon signalling alters the microglial response to Aβ1–42
13-month APPswePS1ΔE9 × IFNAR1−/− microglia adopt a stellate-like morphology. 30 μm thick brain sections of 9 and 13-month old APPswePS1ΔE9 and APPswePS1ΔE9 × IFNAR1−/− mice were stained with both IBA-1 and WO2 antibodies before 15 μm z-stacks were taken from of the hippocampal region. Representative images are shown for 13-month APPswePS1ΔE9 and APPswePS1ΔE9 × IFNAR1−/− in (a) and (b) respectively. A minimum spanning tree algorithm was then employed to measure morphological characteristics with an overview of the skeletonising process in (c) and (d) for both genotypes respectively. Measurements are then shown for (e) total branch length, (f) cell area and (g) cell radius. Data is displayed as mean ± SEM (*p < 0.05, Students t-test, APPswePS1ΔE9 vs. APPswePS1ΔE9 × IFNAR1−/−, n = 3–8). Scale bar = 500 μm.
