Figure 4
Sequencing and flow cytometry-based comparison of enrichments. Panel A shows community characterization by high-throughput amplicon sequencing, while panel B shows flow cytometric characterization of enriched communities using flow cytometric fingerprinting. Both panels show the principle coordinate analysis (PCoA) based on the Bray-Curtis distance metric between samples. pH and inoculum are indicated by color and shape of the points respectively. Black symbols represent communities enriched at pH 5.5, gold points show those enriched at pH 7. Symbol shapes refer to the initial inoculum the community was enriched from; full square for Lindemans brewery full-scale UASB (Li, ▪), full circle for Van Steenberghe brewery full-scale UASB (VS, •) full triangle for Ossemeersen full-scale waste activated sludge digester (Os, ▲), empty square for goat feces (Go, ▫), empty circle for sheep feces (Sh, ◦), and plus sign for + lab-scale pilot fermenting thin stillage to caproic acid.
