Figure 5
From: Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
Alternative splice variants of Hs-vap1. (a) Alignment of H. glycines Vap1 (AF374388) gene and H. schachtii Vap1 transcript isoforms (Hs-Vap1 and Hs-Vap1a). (b) PCR amplification results. Lanes are grouped by the pairs of primers used for amplification (written on the top of each group) and each lane are marked as (A) lanes where cDNA was used or (D) lanes where DNA was used. N = negative. (c) Expression level of Hs-Vap1 isoforms in 5 and 10 dpi. The forward primers are mentioned above each lane. Lane M: 1Kb DNA marker.
