Figure 4 | Scientific Reports

Figure 4

From: Metformin-induced suppression of IFN-α via mTORC1 signalling following seasonal vaccination is associated with impaired antibody responses in type 2 diabetes

Figure 4

Metformin (but not glibenclamide) reduced IFN-α expression in human monocytes after seasonal vaccination. Monocytes from individuals without DM (non-DM; n = 5) were treated with metformin (Met) or glibenclamide (GB) or a combination of both for 30 min. Drug-treated monocytes were incubated with poly I:C or TIV (influenza split-virion) for 3 h before RNA extraction and determination of IFN-α expression by real-time PCR. Expression is shown as fold expression. Each point represents an individual. Statistical analyses were undertaken using one-way ANOVA. Horizontal lines represent the median with interquartile range. ****p < 0.0001; ns, not significant.

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