Table 2 Restriction enzymes sites on DNA.
Probe | Name | Genomic DNA digest | WT allele (kb) | Targeted Allele (kb) |
|---|---|---|---|---|
Neo | 5′ arm first digestion | Afl III | / | 12.3 |
5′ arm second digestion | Eco NI | / | 11.7 | |
5′ arm third digestion | Eco RV | 5.7 | ||
GFP | 3′ arm first digestion | Kpn I | / | 9 |
3′ arm second digestion | Xba | / | 7.7 | |
3′ arm third digestion | Pac I | / | 10.9 | |
5′ external | first digestion | Sex AI | 6.7 | 14.7 |
3′ external | first digestion | Eco RI | 15.6 | 13.8 |
second digestion | Eco RV | 11.5 | 13.8 | |
Third digestion | Hinc II | 9.3 | 12.1 |
