Figure 2 | Scientific Reports

Figure 2

From: Inhibition of Grb14, a negative modulator of insulin signaling, improves glucose homeostasis without causing cardiac dysfunction

Figure 2

Grb14 Knockdown Improves Glucose Homeostasis. (A) Ad lib plasma insulin during an 18-week high-fat diet (HFD) in C57BL/6J male mice (n ≥ 6). (B) 6h-fast plasma insulin during an 18-week HFD in C57BL/6J male mice (n ≥ 6). (C) Overnight-fast plasma insulin during a 20-week HFD in C57BL/6J male mice (n ≥ 6). (D) 6h-fast blood glucose during an 18-week HFD in C57BL/6J male mice (n ≥ 6). (E) Oral glucose tolerance test after a 16-week HFD in C57BL/6J male mice (n ≥ 6). AUC: area under curve. (F) QPCR analysis of gene expression in the liver after a five-month HFD in C57BL/6J male mice (n ≥ 6). (G) QPCR analysis of gene expression in the heart after a five-month HFD in C57BL/6J male mice (n ≥ 6). (H) QPCR analysis of gene expression in the epididymal white adipose tissue (eWAT) after a five-month HFD in C57BL/6J male mice (n ≥ 6). *P < 0.05, **P < 0.01 Empty Vector vs Grb14 shRNA, Two-way ANOVA for (A–D), Student’s t-test for (E–H).

Back to article page