Figure 2

Acute metabolic disturbance induced by K/X anaesthesia. Liver samples were obtained from control and K/X mixture treated mice at 3 h after anesthesia. (A) Glycogen deposition was evaluated by PAS staining. Upper, magnification: 100× and scale bar: 200 um. Steatosis was assessed by ORO staining. Bottom, magnification: 400× and scale bar: 50 um. (B) PLS-DA of untargeted metabolomics spectra from liver tissues collected before and post K/X treatment. Two dimensional PLS-DA score plot revealed separation in metabolite profile induced by K/X anesthesia. Ellipses represented the 95% confidence interval. (C) Changes in metabolites levels was illustrated using heatmap. Each row represents a liver sample and each column represents the expression level of a metabolite. Data are expressed as fold change between control and K/X-treated mice and shown in a color-coded way. Red color represents an increase, and blue color a decrease. (n = 5/group). (D) CT and FDG-PET scan were performed prior to, at 3 h and 24 h post K/X treatment and showed tracer retention in the liver (outline by white dotted line), especially at time of three hour. (E) Quantitative analysis by SUVmean value showed a profound increase of FDG signal at 3 h, but not at 24 h post K/X treatment (n ≥ 5/group, ****P < 0.0001).