Figure 6

Raman spectra of an individual optically-trapped POPC/Chol liposomes (1:1 mol ratio), in the region of the C–H stretching band, recorded at 30 s intervals following exposure to (a) PLY, (b) the PLY D4 and the point mutants (c) N339R and (d) D205R. Only the raw experimental spectra recorded after 0 s and 720 s are shown. Analysis of the full set of spectra for PLY are shown in Fig. 1, for D4 and N339R in the Supporting Information (S6), and for D205R in Fig. 7.