Figure 1
From: Generation of human androgenetic induced pluripotent stem cells
Characterization of human androgenetic induced pluripotent stem cells. (a) Morphology of AgFibs and AgHiPSCs, and alkaline phosphatase (AP) staining of AgHiPSCs. Scale bars = 100 μm. (b) Immunocytochemistry for pluripotency markers. Scale bars = 100 μm. (c) qRT-PCR analysis of the expression of OCT4, SOX2, and NANOG in AgFibs and AgHiPSCs, compared with the H9 positive control. The expression levels in H9 were set to 1. Data are shown as mean ± SEM (n = 3). Significance of the differences between cell types was determined by t-test, and p-values < 0.001 are indicated with ***. Ns means not significant. (d) Bisulfite sequencing analysis of OCT4 promoter regions in AgFibs, AgHiPSCs, and H9 cells. Each line represents a separate clone. Black and white circles represent hypermethylated and hypomethylated CpGs, respectively.
