Figure 4
From: Comparison of Target Enrichment Platforms for Circulating Tumor DNA Detection
The sensitivity of detecting spike-in variants at different read depth. Libraries prepared with 15 ng of cfDNA spiked in with (a) 0.1%, (b) 1%, (c) 10% and (d) 50% of cfDNA reference were sequenced with ¼ lanes of NextSeq High Output kit. Read pairs were normalized by random sampling at 50–350 M read pairs with 50 M increment.
