Figure 2
From: Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Schematic of CDL-PCR. (1) Genomic DNA was fragmented separately by a group of restriction enzymes, generating restriction fragments with same overhang ends. (2) Type I of circularization and multimerization, harboring both flanking sequence and known sequence, reduced the success rate of LM-PCR; they were cut again and ligated with adapters to form target ligation products after cyclic digestion and ligation reaction in CDL-PCR. Type II of circularization and multimerization transformed into invalid ligation, giving no product after nested PCR.
