Figure 3
From: Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Adapter preparation and target flanking sequence amplification by CDL-PCR. M1: TAKARA 1-kb DNA ladder; M2: GeneRuler 1-kb DNA ladder; all the primers sequence used here are listed in Table 2. (a) Preparation of adapter2 and adapter6; lane 1 is the adapter precursor sequence (APS) amplified by PCR with primers APS-F and APS-R, lane 2 is adapter2 digested by Sau3AI, and lane 4 is adapter6 digested by HpaII; (b) The second round of PCR products amplified from transgenic rice DR24 and MDH13; lanes 1–3 are PCR products obtained from DR24 with primers LSP2 and AP2, lanes 4–6 are PCR products obtained from MDH13 with primers MSP2 and AP2.
