Table 1 Twelve adapters with different overhangs and the corresponding isocaudomers.
From: Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Adapters | Enzymes used for the preparation of adapters and their recognition sites in APS | Overhang | Corresponding isocaudomers |
|---|---|---|---|
Adapter1 | NgoMIV (G ↓ CCGG ↑ C) | 5′CCGG | XmaI, AgeI, BspEI |
Adapter2 | Sau3AI (↓GATC ↑) | 5′GATC | BamHI, BclI, BglII |
Adapter3 | TatI (A ↓ GTAC ↑ T) | 5′GTAC | Acc65I, BsiWI, BsrGI |
Adapter4 | CviQI (G ↓ TA↑C) | 5′TA | NdeI, AseI |
Adapter5 | BsaI (GGTCTCC ↓ CTAG ↑) | 5′CTAG | XbaI, SpeI, NheI |
Adapter6 | HpaII (C ↓ CG ↑ G) | 5′CG | AclI, ClaI, BstBI, NarI |
Adapter7 | FatI (↓CATG↑) | 5′CATG | NcoI, BspHI, PciI |
Adapter8 | BstXI (CCAG↑TGCA ↓ CTGG) | 3′TGCA | PstI, NsiI, SbfI |
Adapter9 | BbsI (GAAGACTT ↓ CGCG ↑) | 5′CGCG | BssHII, MluI, AscI |
Adapter10 | BsmBI (CGTCTCA ↓ TCGA ↑) | 5′TCGA | XhoI, PspXI, SalI |
Adapter11 | MluCI (↓ AATT ↑) | 5′AATT | EcoRI, MfeI |
Adapter12 | EaeI (T ↓ GGCC ↑ A) | 5′GGCC | NotI, EagI, PspOMI |
