Table 2 Primers used in this study.
From: Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Primer name | Sequence of oligonucleotides (5′-3′) | Application |
|---|---|---|
APS-F | GCGGGCTTCACTGTTCCATTTAG | Amplification of the adapter precursor sequence (APS) |
APS-R | GGCGGCATAGGTTTCCAAGTCCA | |
AP1 | GCACTCGGACGAACGTGT | Nested primers in adapter |
AP2 | GTGCACCTCTGCACGACGACCTGAGCTCAT | |
LSP1 | CACCACAATGGAGGTATGTGAGGTCCGATGTACT | Specific nested primers for DR24 |
LSP2 | GCATCCTCTTGATGAGTAAACCTCTTGAAGTACTG | |
MSP1 | TGATGGACGATTTTCTCTGGGAACGGAT | Specific nested primers for MDH13 |
MSP2 | GCTTGCTGAGAAGAAATGCGTTGCCCA | |
OSP1 | GTAACCGACTTGCTGCCCCGAGAATTATGCAG | Specific nested primers for At-GT75 |
OSP2 | ATGTGGGCCCCAAATGAAGTGCAGGTC | |
DJ-F | CGCTCATGTGTTGAGCATATAAGAAACCCTTAG | Detection primers for DR24 |
DJ-R | CCGTTCTCTAGTCACTTCCACATCGATCTTC | |
MJ-F | ATCTGTCTGTATCGCTCTAAGGCCCCGTTTAG | Detection primers for MDH13 |
MJ-R | GACTGGGAAAACCCTGGCGTTACCCAACT | |
GJ4-F | GTAACCGACTTGCTGCCCCGAGAATTATGCAG | Detection primers for the insertion site on Chr. 4 of At-GT75 |
GJ-R | GAGTATTTCAACTGCCATTTGGGCTGAATTG | |
GJ1-F | ATGTGGGCCCCAAATGAAGTGCAGGTC | Detection primers for the insertion site on Chr. 1 of At-GT75 |
GJ-R | GAGTATTTCAACTGCCATTTGGGCTGAATTG |
