Table 3 T-DNA insertion sites of 12 transgenic rice lines DR25-DR36.
From: Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Transgenic rice lines | Isocaudomers used here | T-DNA insertion site |
|---|---|---|
DR25 | BamHI, BclI, and BglII | Failed |
DR26 | BamHI, BclI, and BglII | Chr.4: 16227727–16227728 |
DR27 | BamHI, BclI, and BglII | Chr.8:18652264–18652264 |
DR28 | BamHI, BclI, and BglII | Chr.6: 27858668–27858669 |
DR29 | BamHI, BclI, and BglII | Chr.1:42534667–42534668 |
DR30 | BamHI, BclI, and BglII | Failed |
DR31 | BamHI, BclI, and BglII | Chr.8: 27008930–27008931 |
DR32 | BamHI, BclI, and BglII | Failed |
DR33 | BamHI, BclI, and BglII | Chr.11: 4605702–4605703 |
DR34 | BamHI, BclI, and BglII | Chr.6: 27858668–27858669 |
DR35 | BamHI, BclI, and BglII | Failed |
DR36 | BamHI, BclI, and BglII | Failed |
DR25 | BstBI, ClaI, and NarI | Chr.7:17617907–17617908 |
DR30 | BstBI, ClaI, and NarI | Failed |
DR32 | BstBI, ClaI, and NarI | Failed |
DR35 | BstBI, ClaI, and NarI | Chr.12: 25858919–25858920 |
DR36 | BstBI, ClaI, and NarI | Chr.5: 8258053–8258054 |
