Figure 2 | Scientific Reports

Figure 2

From: Topical recombinant human Nerve growth factor (rh-NGF) is neuroprotective to retinal ganglion cells by targeting secondary degeneration

Figure 2

Topical rh-NGF promoted RGC soma survival in the retinal whole-mounts. (a–d) Brn-3a labelled RGC distribution maps in Normal retina (a), pONT only (b), pONT + NGF 180 µg/ml (C), and pONT + NGF 540 µg/ml (d). The protective effects of topical rh-NGF on Brn3a+ cell density (cells/mm2) (e), nearest neighbour distance (NND) (f) and Regularity Index (RI) (g). Topical rh-NGF in both doses (180 and 540 µg/ml) significantly promoted RGC survival by enhancing cell density, reducing NND, and remaining RI. pONT induced a significant damage of RGCs in the three parameters (RGC density, NND, and RI), compared to normal control. Results are means ± SEM. All stats are one-way ANOVA with Bonferoni post-test, ###p < 0.001: normal control compared to pONT; *p < 0.05, **p < 0.01, ***p < 0.001: NGF treatments compared to pONT. N ≥ 5. Scale bar: 2 mm.

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